Observing xylem and phloem using a light microscope: practical
I can use a light microscope to observe xylem and phloem, and calculate the actual size of the structures.
Observing xylem and phloem using a light microscope: practical
I can use a light microscope to observe xylem and phloem, and calculate the actual size of the structures.
These resources will be removed by end of Summer Term 2025.
Lesson details
Key learning points
- The parts of a light microscope and their functions.
- The method for using a light microscope to observe xylem and phloem, including changing the magnification and focus.
- Light microscopy can be used to observe xylem and phloem in a cross-section of a plant shoot, root or leaf.
- The magnification and actual size of structures observed with a microscope can be calculated.
Keywords
Light microscope - an instrument that uses visible light and lenses to magnify a viewed specimen
Magnification - the number of times greater in size the viewed object is compared to its actual size
Xylem - specialised vessels in plants that transport water and mineral ions
Phloem - specialised vessels in plants that transport sugar, amino acids and other nutrients
Common misconception
Incorrectly calculating the total magnification by adding the magnification of the eyepiece lens to the magnification of the objective lens.
The correct way to calculate the total magnification (by multiplying the magnification of the eyepiece lens by the magnification of the objective lens) is practiced.
Equipment
Light microscope, pre-prepared microscope slides of cross-sections of plant shoot or leaf.
Content guidance
- Risk assessment required - equipment
Supervision
Adult supervision required
Licence
This content is © Oak National Academy Limited (2024), licensed on Open Government Licence version 3.0 except where otherwise stated. See Oak's terms & conditions (Collection 2).
Lesson video
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Starter quiz
6 Questions
eyepiece lens
objective lenses
light source
focus wheel
Exit quiz
6 Questions
