Effects of substrate concentration and temperature on rate of enzyme activity
I can explain how the rate of an enzyme reaction is affected by substrate concentration and temperature.
Effects of substrate concentration and temperature on rate of enzyme activity
I can explain how the rate of an enzyme reaction is affected by substrate concentration and temperature.
Lesson details
Key learning points
- The rate of a reaction is the amount of change (e.g. substrate broken down or product made) per unit of time.
- Enzyme reaction rate increases as substrate concentration increases, until an optimum when all active sites are full.
- Enzyme reaction rate increases with temperature due to more collisions, until an optimum when all active sites are full.
- Enzyme reaction rate decreases as temperature increases above the optimum, as the active site becomes denatured.
- Interpretation of graphs showing effects of substrate concentration and temperature on enzyme reaction rate.
Common misconception
Enzymes die rather than denature.
The word denature is clearly introduced and a CfU explores the word die vs denature.
Keywords
Rate of reaction - A measure of how much change occurs per unit of time.
Concentration - A measure of the quantity of dissolved substance.
Optimum - The conditions where maximum rate of reaction occurs.
Denatured - A permanent change in the shape of an enzyme which stops it from working.
Content guidance
- Risk assessment required - equipment
Supervision
Adult supervision required
Licence
This content is © Oak National Academy Limited (2024), licensed on Open Government Licence version 3.0 except where otherwise stated. See Oak's terms & conditions (Collection 2).
Video
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Starter quiz
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6 Questions
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there are fewer successful collisions so the reaction rate is lower.
all active sites are full and reaction rate is as high as it can be.
the enzyme is denatured. The reaction cannot be catalysed.
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Graph B
Graph A