The effect of pH on the rate of an enzyme reaction: practical
I can carry out an investigation into the effect of changing pH on the rate of an enzyme reaction.
The effect of pH on the rate of an enzyme reaction: practical
I can carry out an investigation into the effect of changing pH on the rate of an enzyme reaction.
Lesson details
Key learning points
- Safe use of materials and apparatus.
- Accurate measurement of reactants and reagents.
- Control of temperature using a water bath or electric heater.
- Continuous sampling of reaction mixture and testing with iodine for the presence of starch.
- Sampling at accurately timed intervals.
Common misconception
Mistakes are often made when preparing the practical, for instance which samples go into which test tubes, or carrying out the practical, such as exactly when to take the continuous sample.
The correct approach to preparing and completing this method accurately and carefully is discussed throughout, with quick checks at key points and the opportunity to try it for themselves.
Keywords
Rate of reaction - How fast a chemical reaction occurs.
PH - A measure of how acidic or alkaline a solution is.
Amylase - An enzyme that breaks down starch into maltose (a type of sugar).
Starch - A carbohydrate, which is a polysaccharide (a polymer of sugar).
Continuous sampling - A method that involves taking regular and frequent samples.
Content guidance
- Risk assessment required - equipment
Supervision
Adult supervision required
Licence
This content is © Oak National Academy Limited (2024), licensed on Open Government Licence version 3.0 except where otherwise stated. See Oak's terms & conditions (Collection 2).
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Starter quiz
6 Questions
independent variable
control variable
dependent variable
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