The effect of temperature on the rate of decomposition by an enzyme: practical
I can carry out an experiment to investigate how temperature affects the rate of decomposition of milk by an enzyme.
The effect of temperature on the rate of decomposition by an enzyme: practical
I can carry out an experiment to investigate how temperature affects the rate of decomposition of milk by an enzyme.
Lesson details
Key learning points
- Use an enzyme (e.g. lipase) to decompose milk.
- Measure the change in pH as the milk is decomposed.
- Estimate the rate of decomposition by measuring the change in pH.
- Compare the rate of decomposition at different temperatures.
Common misconception
Several liquids are measured in this practical. The equipment used to measure the lipase should not be used to measure the liquids or the lipase may begin to digest fats before the practical starts.
To prevent contamination, ensure that the method is explained carefully and modelled through a demonstration or by watching the practical video.
Keywords
Enzyme - A protein which acts as a biological catalyst. This speeds up a reaction without being used up.
Active site - The part of an enzyme where the substrate binds.
PH - A measure of how acidic or alkaline a solution is.
End point - The point at which a reaction is complete, often indicated by a change in colour of an indicator.
Hypothesis - A precise, measurable and testable statement based on observations about how something works.
Content guidance
- Risk assessment required - equipment
Supervision
Adult supervision required
Licence
This content is © Oak National Academy Limited (2024), licensed on Open Government Licence version 3.0 except where otherwise stated. See Oak's terms & conditions (Collection 2).
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