New
New
Year 10
Edexcel
Higher

Observing mitosis in plant cells using a light microscope (including PMAT)

You can use a light microscope to observe and identify plant cells in different stages of mitosis.

New
New
Year 10
Edexcel
Higher

Observing mitosis in plant cells using a light microscope (including PMAT)

You can use a light microscope to observe and identify plant cells in different stages of mitosis.

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Lesson details

Key learning points

  1. A light microscope can be used to observe plant cells in different stages of mitosis (e.g. from onion root tip).
  2. The parts of a light microscope and their functions.
  3. The sequence of steps to set up a light microscope to observe cells, including changing the magnification and focus.
  4. Explaining observations from microscopy using ideas about what happens during the cell cycle (including PMAT).

Keywords

  • Light microscope - A type of microscope that uses visible light and a system of lenses to generate magnified images of small objects.

  • Mitosis - A type of cell division that produces genetically identical cells.

  • Lens - A piece of glass or other transparent material with curved sides, used in a microscope to magnify objects.

  • Magnification - Making small objects appear larger in order to see more detail.

  • Focus - A point where light rays converge to form an image with clarity.

Common misconception

Recognising the difference between images of interphase and prophase. In particular, the idea of DNA condensing to form visible chromosomes during prophase.

There are two slides that aim to show the difference between DNA held within the nucleus and visible chromosomes where DNA has condensed. There is also a check for understanding question and practise task question to reinforce this concept.

Students could use the microscopes to identify the stages of mitosis themselves.
Teacher tip

Equipment

Light microscopes and prepared slides.

Licence

This content is © Oak National Academy Limited (2024), licensed on Open Government Licence version 3.0 except where otherwise stated. See Oak's terms & conditions (Collection 2).

Lesson video

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6 Questions

Q1.
What word describes making objects appear larger using a lens?
focus
Correct answer: magnification
resolution
Q2.
What are the smallest living units of life?
tissues
organs
virus
Correct answer: cells
Q3.
What is this piece of scientific equipment called?
An image in a quiz
Correct Answer: miroscope, light microscope
Q4.
What is mitosis?
A type of cell division that creates four different cells.
The process by which the sex cells are formed.
Correct answer: A type of cell division that creates two genetically identical cells.
Q5.
Put these stages of mitosis into the correct order
1 - interphase
2 - prophase
3 - metaphase
4 - anaphase
5 - telophase
6 - cytokinesis
Q6.
Which of the following is not true for mitosis?
Correct answer: Daughter cells have half the amount of DNA.
Two genetically identical cells are produced.
Multicellular organisms use mitosis for growth and repair.

6 Questions

Q1.
Which part of the microscope is the line pointing towards?
An image in a quiz
eyepiece lens
coarse focus wheel
Correct answer: objective lens
stage
Q2.
In which two places does mitosis take place in an onion?
Correct answer: tips of shoots
outer layer of skin
Correct answer: tips of roots
inner layer of skin
Q3.
Put these stages in order to show how a microscope should be used?
1 - Turn the objective lens to the lowest power.
2 - Clip the specimen onto the stage and turn on the light.
3 - Use the coarse focus wheel to roughly focus the image.
4 - Use the fine focus wheel to get a sharp focus.
5 - Turn the objective lens to a higher power to increase the magnification.
Q4.
The image has circled a stage of mitosis, what stage is this?
An image in a quiz
prophase
Correct answer: metaphase
telophase
anaphase
Q5.
The image has circled a stage of the cell cycle, what stage is this?
An image in a quiz
prophase
Correct answer: cytokinesis
telophase
anaphase
Q6.
Which image has cirled interphase?
Correct Answer: An image in a quiz
An image in a quiz
An image in a quiz
An image in a quiz
Q4 Dimarion/shutterstock Q5 Dimarion/shutterstock Q6 image 1 Dimarion/shutterstock Q6 image 2 Rattiya Thongdumhyu/shutterstock Q6 image 3 Dimarion/shutterstock