Using a microscope to observe effects of osmosis in plant cells: practical
I can use a light microscope to observe effects of osmosis in plant cells.
Using a microscope to observe effects of osmosis in plant cells: practical
I can use a light microscope to observe effects of osmosis in plant cells.
Lesson details
Key learning points
- Use a light microscope to observe effects of osmosis in plant cells (including: shrinking/crenation, swelling/bursting)
- The parts of a light microscope and their functions.
- The sequence of steps for setting up a light microscope to observe cells, including changing magnification and focus.
- Explaining observations from microscopy using ideas about osmosis and net movement of water into/out of cells.
Common misconception
The process of osmosis, the direction of movement from high to low water concentration, and the effect that this has on plant cells in terms of turgidity and flaccidity are frequently confused.
Errors are explored, explained and questioned, and clear demonstrations of turgidity and flaccidity and the effects that these have on a plant are demonstrated. This will be reinforced by observation of these processes using a light microscope.
Keywords
Light microscope - an instrument that uses visible light and lenses to magnify viewed objects
Selectively-permeable membrane - a membrane that will only allow some substances through and not others
Osmosis - the net movement of water molecules from high to low concentration through a selectively-permeable membrane
Turgid - a cell which is swollen with water and firm
Flaccid - a cell which has lost a lot of water and is soft and limp
Equipment
light microscope, slides with onion epidermis in states of turgor and plasmolysis
Content guidance
- Risk assessment required - equipment
Supervision
Adult supervision required
Licence
This content is © Oak National Academy Limited (2024), licensed on Open Government Licence version 3.0 except where otherwise stated. See Oak's terms & conditions (Collection 2).
Video
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Starter quiz
6 Questions
provides support and structure
controls what enters and leaves the cell
the site of photosynthesis
contains cell sap (water and other substances)
Exit quiz
6 Questions
eyepiece lens: viewing lens with ×10 magnification
objective lenses: three lenses with different magnifications
stage: specimen on a slide is placed here
light source: illuminates the specimen so that it can be observed
coarse focus wheel: for adjusting the focus in larger increments
fine focus wheel: for adjusting the focus in smaller increments
