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Year 7

Chromatography

I can describe how chromatography works and use a chromatogram to answer questions about a substance.

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New
New
Year 7

Chromatography

I can describe how chromatography works and use a chromatogram to answer questions about a substance.

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These resources were created for remote use during the pandemic and are not designed for classroom teaching.

Lesson details

Key learning points

  1. Chromatography can be used for both separation of minute samples and analysis.
  2. A pure substance will only produce one spot on a chromatogram.
  3. A mixture will produce many spots on a chromatogram.
  4. Comparing chromatograms can help to identify an unknown component in a sample.
  5. Pencil is insoluble in water.

Keywords

  • Solvent - A solvent is a liquid into which a solute dissolves.

  • Soluble - When a substance dissolves in a liquid, it is described as soluble in that liquid.

  • Chromatography - Chromatography is a technique that separates a mixture into its different parts and the results can be analysed.

  • Chromatogram - A chromatogram is the resultant pattern on the stationary phase after chromatography has been carried out.

  • Compare - To compare is to look for similarities and/or differences between two objects or ideas.

Common misconception

Pupils can struggle to interpret information that contain several samples on one chromatogram.

Stress a systematic approach to interpreting chromatograms - use rulers. Live model this - using a visualiser helps enormously.


To help you plan your year 7 science lesson on: Chromatography, download all teaching resources for free and adapt to suit your pupils' needs...

Create a mini-crime scene (e.g. stolen plant with a ransom note) and have pupils conduct chromatography on different pens to identify who wrote the note. Don't forget to make a chromatogram of the ransom note pen beforehand to use for comparison!
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Adult supervision required

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Licence

This content is © Oak National Academy Limited (2025), licensed on Open Government Licence version 3.0 except where otherwise stated. See Oak's terms & conditions (Collection 2).

Lesson video

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6 Questions

Q1.
If you have a mixture of sand and salt, adding water will dissolve the salt. What will happen to the sand?
An image in a quiz
It will dissolve.
Correct answer: It will sink to the bottom of the container.
It will evaporate out of the container.
Q2.
A substance that dissolves in a solvent is said to be in that solvent.
Correct Answer: soluble
Q3.
Match the word with the definition.
Correct Answer:Absorb,Ability of a substance to take in another substance.
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Ability of a substance to take in another substance.

Correct Answer:Solute,A substance that can dissolve in a liquid.
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A substance that can dissolve in a liquid.

Correct Answer:Solvent,A liquid in which a solute dissolves.
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A liquid in which a solute dissolves.

Correct Answer:Sample,A substance which will be tested in an investigation.
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A substance which will be tested in an investigation.

Correct Answer:Miscible,Ability of a liquid to dissolve/mix in another liquid.
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Ability of a liquid to dissolve/mix in another liquid.

Q4.
Order the following steps describing how to make a solution.
1 - If necessary, crush the solute into small pieces.
2 - Add the solute to the solvent.
3 - Stir to dissolve the solute.
4 - Filter the mixture to remove any undissolved solute.
Q5.
Oil and water are two liquids that cannot mix. They are said to be ...
An image in a quiz
Correct Answer: immiscible
Q6.
Which of the following substances are mixtures?
Correct answer: Pure orange juice
Copper sulfate
Correct answer: Ink
Oxygen
Correct answer: Tap water

6 Questions

Q1.
What is chromatography used for?
To mix coloured substances together.
Correct answer: To separate and analyse coloured mixtures.
To create coloured solutions.
Q2.
The pattern of results obtained from chromatography is called a ...
Correct Answer: chromatogram, chromatogram.
Q3.
How does a pure substance behave in chromatography?
It produces multiple spots.
It does not move.
Correct answer: It produces one spot only.
Q4.
The line on the chromatogram on which the samples are placed must be drawn in pencil because it is in the solvent.
Correct Answer: Insoluble
Q5.
Order these steps in performing chromatography, starting with drawing a pencil line.
1 - Draw a pencil line 1 cm up from the bottom of a piece of chromatography paper.
2 - Carefully add a small drop of your sample to the pencil line.
3 - Place a small volume of solvent in a beaker, lower than 1cm depth.
4 - Wind your paper on a support so that the pencil line will be above the solvent.
5 - Lower your paper vertically into the beaker so it just touches the solvent.
6 - Leave to allow the solvent to absorb up the paper and separate your sample.
7 - Remove and allow to dry.
Q6.
What can you conclude from this chromatogram?
An image in a quiz
Dye A contains three substances.
Correct answer: Dye B is insoluble in the solvent used.
Correct answer: Dye C contains only one substance.
Correct answer: Dye A is a mixture.
Dye C contains the most soluble substance.
Q6 UYSEG